Dataset 669

Phytoplankton Lake Bidighinzu

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Realm: Freshwater
Climate: Temperate
Biome: Small Lake Ecosystems
Central latitude: 40.556059
Central longitude: 8.661328
Duration: 16 years, from 1988 to 2015

3795 records

220 distinct species

Across the time series Oocystis is the most frequently occurring species

Methods

The samplings were carried out at a representative point of the system, positioned near the dam at a safe distance. The samples were taken with a Niskin bottle at the limnologically characterizing depths along the vertical profile in the photic zone and fixed in situ with Lugol's solution. Samples for phytoplankton analysis were collected from the photic zone at the selected depths (generally at 0, 1, 2.5, 5, 7.5, 10 m). To fix the phytoplank-ton samples, Lugol's iodine solution was used, and analysis was conducted after the sedimentation of varying water volumes (5-10 ml, mostly 10mL), depending on the cell density of phytoplankton. An inverted microscope (Zeiss, Axiovert 25) was employed for cell counting, with the entire bottom of the sedimentation chamber observed at 200× magnification for larger and easily identifiable species. A selected number of fields were further examined at 400× magnification for smaller cells. Species identification was accomplished using several taxonomic guides as mentioned in Mariani et al. 2015. Unidentified cells within the size range of 5-10 µm were classified as nano-plankton and denoted as "undetermined" and are not part of this dataset.

Citation(s)

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