Dataset 805
Bahía Blanca Estuary Puerto Cuatreros - Phytoplankton (2008-2010)
Realm: Marine
Climate: Temperate
Biome: Temperate Shelf and seas ecoregions Central latitude: -38.828380
Central longitude: -62.249250
Duration: 3 years, from 2008 to 2010
Climate: Temperate
Biome: Temperate Shelf and seas ecoregions Central latitude: -38.828380
Central longitude: -62.249250
Duration: 3 years, from 2008 to 2010
308 records
36 distinct species
Across the time series Flagellate is the most frequently occurring speciesMethods
The composition and abundance of protistan phytoplankton (>5 µm) and microzooplankton (phagotrophic protists with cell size between 20 and 200 ?m, mainly tintinnids and oligotrichs) have been extensively studied by light microscopy in the Bahía Blanca Estuary during discontinuous periods from 1978 to 2019 on a biweekly/monthly sampling basis (e.g. Gayoso 1999; Guinder et al., 2010), or more sporadic in the last decade (2011-2019) (e.g. Berasategui et al., 2023). Together with the sustained observations of protistan plankton, in situ sea surface temperature, salinity, chlorophyll a and dissolved inorganic nutrients (silicates, DIN and phosphate) have been continuously measured on a biweekly basis since 1978. The high frequency monitoring allowed the characterization of the phenology and biodiversity of the phytoplankton blooms in relation with changing environmental conditions (Gayoso 1981; 1989; 1998; Popovich and Gayoso 1999, Popovich et al. 2008; Guinder et al. 2010; 2012; 2013; 2015; 2016; 2017; López Abbate et al., 2017; Berasategui et al., 2021); and similarly with the microzooplankton (Pettigrosso 2003; Pettigrosso and Popovich 2009; Barría de Cao 2005; 2011; López Abbate et al. 2016; 2019a; 2019b). It is worth noting that collection and processing of samples have been consistent throughout the study period, including the identification and quantification of species under light microscopy. This high resolution plankton survey has been performed by the same group of specialists, ensuring that changes in the community structure and/or composition are due to environmental dynamics and not attributable to analytical processes. Samples were collected at a fixed station (Puerto Cuatreros, 38.50S; 62.20W), a shallow harbor (mean depth of 6 m) at the head of the Bahía Blanca Estuary. Samples were collected at high tide and around noon time from a pier. For phytoplankton and microzooplankon cells enumeration, surface water samples were taken using a Van Dorn bottle (2.5 l) and preserved in acidified Lugols solution. For qualitative analysis, samples were collected using a Nansen 30 µm net and preserved with formaldehyde (final concentration 0.4%). The phytoplankton abundance (in cells L-1) was determined using a Sedgwick-Rafter chamber (1 mL) under light microscopy from 1978-2008 (400x), and Utermöhl chambers under light inverted micrsocopy. The entire chamber was examined at 400x magnification, and each cell was counted as a unit (McAlice, 1971). Phytoplankton was identified at species level using a Zeiss Standard R microscope and a Nikon Eclipse microscope, with magnification of 1000x and phase contrast. In some cases, scanning electron microscopy was performed to identify diatom species. The quantification of microzooplankton (e.g. ciliates and heterotrophic dinoflagellates, as well as phytoplankton to contrast methodologies) was done by settling a variable volume (1050 ml, depending on sediment and plankton concentration) of preserved seawater sample in Utermöhl chambers during 24 h. The entire chamber was analysed under a Wild M20 inverted microscope (Hasle, 1978). The units of Abundance are cell µl-1, the units of Biomass are µgC l-1.Citation(s)
.
In (Eds.),
(p. ).
:
.
,
(),
.
